简历详情

Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA

ABSTRACTHuman induced pluripotent stem cell (h-iPSC)–derived endothelial cells (h-iECs) have become a valuable tool in regenerative medicine. However, current differentiation protocols remain inefficient and lack reliability. Here, we describe a method for rapid, consistent, and highly efficient generation of h-iECs. The protocol entails the delivery of modified mRNA encoding the transcription factor ETV2 at the intermediate mesodermal stage of differentiation. This approach reproducibly differentiated thirteen diverse h-iPSC lines into h-iECs with exceedingly high efficiency. In contrast, standard differentiation methods that relied on endogenous ETV2 were inefficient and notably inconsistent. Our h-iECs were functionally competent in many respects, including the ability to form perfused vascular networks in vivo. Importantly, timely activation of ETV2 was critical, and bypassing the mesodermal stage produced putative h-iECs with reduced expansion potential and inability to form functional vessels. Our protocol has broad applications and could reliably provide an unlimited number of h-iECs for vascular therapies.

作者: Juan M. Melero-Martin,George M. Church,William T. Pu,Minglin Ma,Xi Wang,Gang Wang,Joseph Neumeyer,Chin Nien Lee,Alex H. Ng,Xuechong Hong,Ruei-Zeng Lin,Kai Wang
DOI:10.1101/2020.03.02.973289

A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice

期刊: Biomaterials  2017
作者: Ying Luo,Ming Qiang,Wei Zhang,Kai Wang,Xi Wang
DOI:10.1016/j.biomaterials.2017.05.033

From Micro to Macro: The Hierarchical Design in a Micropatterned Scaffold for Cell Assembling and Transplantation

期刊: Advanced Materials  2016
作者: Ying Luo,Liangyi Chen,Jinyang Wang,Wenda Hou,Chengsheng Han,Xi Wang,Kai Wang
DOI:10.1002/adma.201604600

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

期刊: Biomaterials  2016
作者: Ying Luo,Liangyi Chen,Qiu-Shi Ren,Wen-Xi Zhang,Ni Su,Ivan Vuletic,Chengsheng Han,Xi Wang,Wen-Da Hou,Kai Wang
DOI:10.1016/j.biomaterials.2016.06.028